It began at Coruscant and passed the worlds of Anaxes, Corulag, Brentaal IV, Carida, Nak Shimor, Taanab, Kulthis, Rhen Var, Sorjus, Vaynai, Makem Te, Ter Abbes, and terminated just past Quermia. It also passed the Roche asteroid field, The Wheel, and the Cron Drift.
The Route first connected Coruscant to Ossus, and Coruscanti reunited with the Jedi, the ascetic warriors who left the Core centuries ago and remained but a myth until then. The Route contributed to the growth of the Republic since civilizations and colonies sued for protection from the Tionese and Hutt Space. One unfortunate consequence of the discovery of routes such as the Perlemian Trade Route and their increased use was the increased obsolescence of large hyperspace hubs throughout the Mid and Outer Rims, and the accompanying impact such loss of commerce would have upon nearby systems.
Perhaps the most infamous example of this (to date) was the decline of the planet Taris after the Perlemian's increased use a couple of decades after the Great Sith War. With an increasing amount of traffic getting diverted to the hyperlane, Taris soon began a steep slide into decay, which would last until the planet's destruction by the Sith in the year 3956 BBY.
With the Corellian Run, it formed The Slice region of the Galaxy which was explored and populated easier and faster than other parts. Further expansion of the Route led it up to the Wild Space.
Cardan-class space stations were present at the crossroads between the Perlemian Trade Route and the Hydian Way.